Studii clinice care confirma calitatile acestui produs

• Ikeda, et. al. Intake of Fermented Soybeans, Natto, Is Associated with Reduced Bone Loss in Postmenopausal Women: Japanese Population-Based Osteoporosis (JPOS) Study J. Nutr. 136: 1323–1328, 2006.

• Jeleinjse, et. a. Dietary Intake of Menaquinone Is Associated with a Reduced Risk of Coronary Heart Disease: The Rotterdam Study. J. Nutr. 134: 3100–3105, 2004.

• Kaneki M et al : Japanese fermented soybean food as the major determinant of the large geographic differences in circulating levels of vitamin K2: Possible implications for hip-fracture risk. Nutrition. 2001, 17: 315-321.

• Schurgers, et. al. K1 and natto-derived menaquinone-7. Blood First Edition Paper, prepublished online December 7, 2006; DOI 10.1182/blood-2006-08-040709.

• Schurgers, et al. Regression of warfarin-induced medial elastocalcinosis by high intake of vitamin K in rats. Blood First Edition Paper, prepublished online November 30, 2006; DOI 10.1182/blood-2006-07-035345.

• Vermeer ,C et al: Vitamin K supplementation: A simple way to improve bone and vascular health. AgroFood Industry High-Tech. Nov/Dec 2003.

Clinical Research

Dietary intake of menaquinone is associated with a reduced risk of coronary heart disease: the Rotterdam Study.

Geleijnse JM, Vermeer C, Grobbee DE, Schurgers LJ, Knapen MH, van der Meer IM, Hofman A, Witteman JC.

Department of Epidemiology & Biostatistics, Erasmus Medical Center Rotterdam, Rotterdam, The Netherlands.

Vitamin K-dependent proteins, including matrix Gla-protein, have been shown to inhibit vascular calcification. Activation of these proteins via carboxylation depends on the availability of vitamin K. We examined whether dietary intake of phylloquinone (vitamin K-1) and menaquinone (vitamin K-2) were related to aortic calcification and coronary heart disease (CHD) in the population-based Rotterdam Study. The analysis included 4807 subjects with dietary data and no history of myocardial infarction at baseline (1990-1993) who were followed until January 1, 2000. The risk of incident CHD, all-cause mortality, and aortic atherosclerosis was studied in tertiles of energy-adjusted vitamin K intake after adjustment for age, gender, BMI, smoking, diabetes, education, and dietary factors. The relative risk (RR) of CHD mortality was reduced in the mid and upper tertiles of dietary menaquinone compared to the lower tertile [RR = 0.73 (95% CI: 0.45, 1.17) and 0.43 (0.24, 0.77), respectively]. Intake of menaquinone was also inversely related to all-cause mortality [RR = 0.91 (0.75, 1.09) and 0.74 (0.59, 0.92), respectively] and severe aortic calcification [odds ratio of 0.71 (0.50, 1.00) and 0.48 (0.32, 0.71), respectively]. Phylloquinone intake was not related to any of the outcomes. These findings suggest that an adequate intake of menaquinone could be important for CHD prevention.

Regression of warfarin-induced medial elastocalcinosis by high intake of vitamin K in rats

Leon J Schurgers*, Henri M.H Spronk, Berry A.M Soute, Paul M Schiffers, Jo GR DeMey, and Cees Vermeer

Cardiovascular Research Institute (CARIM), Maastricht University, Maastricht, Netherlands

Dept of Internal Medicine, CARIM, Maastricht University, Maastricht, Netherlands

Dept of Pharmacology & Toxicology, CARIM, Maastricht University, Maastricht, Netherlands

VitaK, Maastricht University, Maastricht, Netherlands

Arterial calcification (AC) is generally regarded as an independent risk factor for cardiovascular morbidity and mortality. Matrix Gla-protein (MGP) is a potent inhibitor of AC and its activity depends on vitamin K (VK). In rats, inactivation of MGP by treatment with the vitamin K-antagonist warfarin leads to rapid calcification of the arteries. Here we investigated whether pre-formed AC can be regressed by a VK-rich diet. Rats received a calcification-inducing diet containing both VK and warfarin (W&K). During a second 6-week period, animals were randomized to receive either W&K (3.0 mg/g & 1.5 mg/g, subsequently), a diet containing normal (5 µg/g) or high (100 µg/g) amount of VK (either K1 or K2). Increased aortic calcium concentration was observed in the group that continued to receive W&K, and also in the group changed to the normal dose of VK, AC progressed. Both the VK-rich diets decreased the arterial calcium content by some 50%. Additionally, arterial distensibility was restored by the VK-rich diet. Using MGP antibodies, local VK-deficiency was demonstrated at sites of calcification. This is the first study in rats demonstrating that AC and the resulting decreased arterial distensibility are reversible by high VK intake.

Japanese fermented soybean food as the major determinant of the large geographic difference in circulating levels of vitamin K2: possible implications for hip-fracture risk.

Masao Kaneki, MDa , Stephen J. Hedges , PhDb , Takayuki Hosoi , MDa , Saeko Fujiwara , MDc , Anthony Lyons , FRCSEdd , St.John Crean , MBBS, FDSRCSe , Nobuhiko Ishida , MDf , Mamoru Nakagawa a.


Increasing evidence indicates a significant role for vitamin K in bone metabolism and osteoporosis. In this study, we found a large geographic difference in serum vitamin K2 (menaquinone-7; MK-7) levels in postmenopausal women. Serum MK-7 concentrations were 5.26 ± 6.13 ng/mL (mean ± SD) in Japanese women in Tokyo, 1.22 ± 1.85 in Japanese women in Hiroshima, and 0.37 ± 0.20 in British women. We investigated the effect of Japanese fermented soybean food, natto, on serum vitamin K levels. Natto contains a large amount of MK-7 and is eaten frequently in eastern (Tokyo) but seldom in western (Hiroshima) Japan. Serum concentrations of MK-7 were significantly higher in frequent natto eaters, and natto intake resulted in a marked, sustained increase in serum MK-7 concentration. We analyzed the relation between the regional difference in natto intake and fracture incidence. A statistically significant inverse correlation was found between incidence of hip fractures in women and natto consumption in each prefecture throughout Japan. These findings indicate that the large geographic difference in MK-7 levels may be ascribed, at least in part, to natto intake and suggest the possibility that higher MK-7 level resulting from natto consumption may contribute to the relatively lower fracture risk in Japanese women.